Latest documents http://publikationen.stub.uni-frankfurt.de/index/index/ Mon, 18 Feb 2013 16:26:04 +0100 Mon, 18 Feb 2013 16:26:04 +0100 http://publikationen.stub.uni-frankfurt.de/frontdoor/index/index/docId/28940 High attrition rates of novel anti-cancer drugs highlight the need for improved models to predict toxicity. Although polo-like kinase 1 (Plk1) inhibitors are attractive candidates for drug development, the role of Plk1 in primary cells remains widely unexplored. Therefore, we evaluated the utility of an RNA interference-based model to assess responses to an inducible knockdown (iKD) of Plk1 in adult mice. Here we show that Plk1 silencing can be achieved in several organs, although adverse events are rare. We compared responses in Plk1-iKD mice with those in primary cells kept under controlled culture conditions. In contrast to the addiction of many cancer cell lines to the non-oncogene Plk1, the primary cells' proliferation, spindle assembly and apoptosis exhibit only a low dependency on Plk1. Responses to Plk1-depletion, both in cultured primary cells and in our iKD-mouse model, correspond well and thus provide the basis for using validated iKD mice in predicting responses to therapeutic interventions. Monika Raab; Sven Kappel; Andrea Krämer; Mourad Sanhaji; Yves Matthess; Elisabeth Kurunci-Csacsko; Julia Calzada-Wack; Birgit Rathkolb; Jan Rozman; Thure Adler; Dirk H. Busch; Irene Esposito; Helmut Fuchs; Valérie Gailus-Durner; Martin Klingenspor; Eckhard Wolf; Nicole Sänger; Florian Prinz; Martin Hrabě de Angelis; Jost Seibler; Juping Yuan; Martin Bergmann; Rainald Knecht; Bertolt Kreft; Klaus Strebhardt article http://publikationen.stub.uni-frankfurt.de/frontdoor/index/index/docId/28940 Mon, 18 Feb 2013 16:26:04 +0100 http://publikationen.stub.uni-frankfurt.de/frontdoor/index/index/docId/27091 Wir beschreiben eine ungewöhnliche Brutkolonie des Mauerseglers unter einer Autobahnbrücke. Im Jahr 2007 bestand die Kolonie aus 38 Brutpaaren, von denen 35 erfolgreich brüteten. Trotzdem war die Überlebensrate der Nestlinge wegen der schlechten Wetterbedingungen sehr niedrig. Nur 7 von 75 Nestlingen flogen aus. Der Standort bietet exzellente Möglichkeiten, weitere Aspekte der Life-History von Mauerseglern zu untersuchen. Mark Walker; Jan Rozman; Klaudia Witte article http://publikationen.stub.uni-frankfurt.de/frontdoor/index/index/docId/27091 Fri, 07 Dec 2012 08:14:37 +0100